Treatment of critical-size bone defect is a great difficulty in orthopedics. Osteogenesis and angiogenesis are critical issue during the process of bone repair and remodeling. MSCs-derived small extracellular vesicles (sEVs) show desirable therapeutic prospects in tissue regeneration due to satisfied advantages including high stability, facilitated acquisition. However, the effect of Human umbilical cord MSCs-derived sEVs (hUC-MSCs-sEVs) on vascularized bone regeneration and the potential mechanism remains to be investigated. Herein, we aimed to explore the therapeutic effect and the mechanism of hUC-MSCs-sEVs on critical-size bone defect. The results showed that hUC-MSCs-sEVs facilitated calcium deposition and the endothelial network formation, inducing osteogenic differentiation and angiogenesis by delivering miR-23a-3p<i> in vitro</i>. The up-regulated miR-23a-3p in cells targeted 3’UTR of PTEN and suppressed the expression of PTEN, resulting in the activation of AKT signaling pathway. Furthermore, we fabricated 3D-printed bioglass (BG) scaffold with Gelma/nanoclay hydrogel (BG-gel-sEVs) coatings to load sEVs. <i>In vivo</i> results revealed that BG-gel-sEVs composited scaffold effectively improved angiogenesis and osteogenesis, enhancing bone regeneration. Consequently, BG-gel-sEVs may provide novel promising strategies to repair critical-size bone defect.

Small extracellular vesicles released from bioglass/hydrogel scaffold promote vascularized bone regeneration by transferring miR-23a-3p

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ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.