Glial cells use ATP as the most relevant signaling molecule for communication between the brain, sensory neurons, and spinal cord. Defective ATP release has been linked to brain injuries, epileptic seizures, strokes, and Alzheimer’s disease. Although various findings support that glial transmission is an important part of the synaptic region of the brain triad, many reports indicate that glial transmission does not occur under physiological conditions but occurs under pharmacological phenomena. This study outlines the fabrication of sub-cellular E-AB-microsensors with the required, sensitivity, specificity, and spatiotemporal resolution to monitor the release of ATP at a single cell. In this in-vitro study, square wave voltammetry (SWV) was used as an interrogation technique to monitor ATP release from astrocyte cells. Our sensors responded with seconds-resolution to changes in intracellular ATP concentration under physiological conditions.  The concentration of intracellular ATP detected by our sensors was quantitatively dependent on the cell population density. At the single cell level, the concentration of ATP detected was dependent on the proximity of neighboring cells in the media. Our sub-cellular EAB-sensors were stable and robust during the duration of our experiment, hence remedying the issue of biofouling in cell media. Our sensor exhibited baseline signals in the presence of the control PC12 cells, indicating the specificity of the sub-cellular EAB sensors

Instantaneous detection of intracellular Adenosine triphosphate (ATP) release from astrocyte cells using sub-cellular electrochemical aptamer based (E-AB) sensors

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.