The OECD 307 Guideline ‘Aerobic and Anaerobic Transformation in Soil’ and the EPA Guideline OCSPP 835.4100 ‘Aerobic Soil Metabolism’ are both standard guidelines for defining the degradation rate of a test substance in a variety of soils. These guidelines and preceding guidelines like them have been used successfully for years. However, questions often still remain concerning the solvent(s) used as a carrier for the test substance applied to the soil in the study and its effect on microbial biomass and the degradation rate of the test substance. A soil metabolism study was conducted by setting up treated samples with [<sup>14</sup>C]Glyphosate, a generic crop protection product, using two different carriers, i.e., aqueous and organic solvent, for the test substance application. Comparisons of the rate of degradation, rate of mineralization, and maintenance of microbial biomass have been made between the soil samples applied with two different solvents. The results of this study provide a justification to continue the use of an appropriate organic solvent as a carrier for the application of the test substance in soil metabolism studies performed with various soil types.

Evaluating the effect of application solvent(s) used during a soil metabolism study with [<sup>14</sup>C]Glyphosate

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Evaluating the effect of application solvent(s) used during a soil metabolism study with [14C]Glyphosate

Evaluating the effect of application solvent(s) used during a soil metabolism study with [¹⁴C]Glyphosate