The intestinal mucosal immune system is an essential part of the mucosal immune system due to its sizeable mucosal area and food interaction properties. According to statistics, over 90% of pathogens can be infected the body through mucous. The intestinal mucosal immune homeostasis imbalance is related to immune diseases, including IBD, chronic diseases, and cancer. Nowadays, there are few systematic studies on intestinal mucosal immunity. The primary purpose of this study is to search for Bifidobacteria and Lactobacillus plantarum that can enhance immunity and develop probiotic preparations to prevent intestinal diseases. During the whole experiment, the experimental group mice were given probiotic preparations for 14 days and intraperitoneally injected LPS (5 mg/kg) to construct an intestinal injury model. Untreated mice were used as the control group to explore the mechanism of the intestinal mucosal immune system. L. plantarum KLDS1.0386 and intestinal Bifidobacterium longum BL-10 were co-cultured to prevent LPS-induced intestinal injury. The results showed that compared with the LPS group, the mix group significantly reduced tissue damage in the jejunum, ileum, and colon, prevented the decrease of mRNA expression of Claudin-1, Occludin, ZO-1, and mucin 2 in the ileum, increased the immune cells CD4+ T cells and IgA plasma cells numbers and significantly prevented the increase of tumor necrosis TNF-α, IL-6, IL-12 and myeloperoxidase contents in the ileum. In addition, the mix group regulated the ratio of Th1/Th2 and Th17/Treg, down-regulated the activation of TLR4 and NF-κB signaling pathway, regulated the structure of gut microbiota, and improved metabolism of metabolites, especially short acid and bile acids. In conclusion, the L. plantarum KLDS1.0386 and B. longum BL-10 colonization may adjust gut microbiota functions, metabolism of nutrients and food components, lower NF-B signal pathway, and inflammatory factor release, increase intestinal immune cells, and improve intestinal immune function.

Immunomodulatory effects of the mixed Lactobacillus plantarum and Bifidobacterium longum on lipopolysaccharide-induced intestinal injury in mice

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.