Holographic particle characterization yields the diameter of individual colloidal spheres with nanometer precision and therefore can resolve probe beads growing as molecules bind to their surfaces. We use this technique to demonstrate label-free bead-based holographic assays for antibodies and for antigenic proteins from pathogenic viruses, including SARS-CoV-2 and H1N1 influenza virus. These studies not only introduce holographic binding assays as a new platform for rapid and cost-effective diagnostic testing, but also quantitatively validate the effective-medium analysis of molecular-scale coatings on colloidal probe beads.

Holographic Molecular binding assays

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.