Lipid bilayers are found in all life on Earth -- they are the effective semipermeable barriers that delineate all cells. Despite only being a few nanometers thick, they are able to encapsulate solutes across several orders of magnitude in size. Consequently, understanding the compartmentalization properties of lipid bilayer vesicles/liposomes is critical in fields as diverse as drug delivery and astrobiology.<br/>In this talk, we discuss how we use digital holographic microscopy to quantify various lipid vesicle/liposome properties. By fitting light scattering models to holograms of vesicles, we are able to quantify vesicle loading, and perform microrheology inside the vesicles. Holographic imaging enables us to avoid the use of fluorescent molecules, which can often interact undesirably with the lipid bilayers.

Holographic characterisation of liposomes/vesicles

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.