In this contribution, a stress-tolerant, recyclable, and autonomously renewable biocatalyst platform based on T7 RNA polymerase-enabled high-density protein display on bacterial spores (TIED) will be discussed. TIED uses high-level T7 RNA polymerase-driven expression of recombinant proteins specifically in sporulating cells to allow spontaneous assembly of recombinant fusion proteins on <i>B. subtilis</i> spore surface. TIED brings spore-based enzyme display to the next level by increasing the expression level and catalytic conversion by orders of magnitude and enables biocatalysis in a traditionally harsh environment. Importantly, TIED allows recycling and autonomous renewal upon attrition of their activity. This work advances the current capabilities in immobilized enzymes by enabling unlimited reuse of catalytic functionality.

Stress-tolerant, recyclable, and autonomously renewable biocatalyst platform enabled by engineered bacterial spores

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.