Tautomerase superfamily (TSF) members repurpose a simple β-α-β unit to make a collection of structures (homo-, heterohexamers, and trimers) that carry out an assortment of activities (primarily tautomerization, dehalogenation, hydration, and decarboxylation reactions). Recently, an asymmetric trimer was discovered where one monomer is flipped 180° relative to the other two monomers to create three different active sites. Since these trimers might provide an additional scaffold for diversification (along with the symmetric trimer), the basis and origins for the asymmetry were investigated. The results show (1) the number of salt bridges governs whether a trimer is symmetric (in A) or asymmetric (in B) and (2) suggest that fusion of an asymmetric heterohexamer led to an asymmetric trimer.

Basis and origin for asymmetric trimers in the tautomerase superfamily: Analysis and implications

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.