Biosensors have shown great promise for use in point-of-care diagnostics and continuous health monitoring. There is a great need for biosensors that are easy to use and do not rely on reagent addition, sample processing, and washes that are difficult to implement in situ or for use by non-technical users. Here, I will discuss strategies focused on the integration of functional nucleic acids with electrochemical readout for reagent-less and wash-free biosensing. This talk will focus on the engineering of redox DNA-barcoded DNAzymes and aptamers that translate the capture of infectious disease targets into the release of a redox barcode. These barcoded functional nucleic acids are immobilized onto electrode or bead surface for biorecognition. The barcodes released from these surfaces are autonomously diffused and analyzed on electrochemical chips for bioanalysis. Our barcoded functional nucleic acids have been demonstrated for the detection of both bacteria (Escherichia coli) and viruses (Porcine epidemic diarrhea virus (PEDv)). These assays have been evaluated for use in unprocessed clinical samples (human urine and porcine saliva) and have demonstrated clinically-acceptable performance.

Barcoded functional nucleic acids for reagent-less biological sensing

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.