Uniform hydrogel microparticles with MOF nanoparticles for molecular co-confinement of cascade enzymes are developed from droplet microfluidics to achieve enhanced stability and reusability under harsh conditions. Water-swollen polyacrylamide hydrogel is used for constructing the microparticles; while microporous zeolitic imidazolate framework-8 (ZIF-8) nanoparticles are incorporated in the hydrogel networks for multi-enzyme co-confinement. With the ZIF-8 nanoparticles as amours, the multi-enzymes in the microparticles enable stable for efficient biocatalytic cascades in hash conditions. This is demonstrated by using glucose oxidase (GOx) and horseradish peroxidase (HRP) as typical cascade enzymes. The hydrogel microparticles with both GOx and HRP confined in their ZIF-8 nanoparticles exhibit enhanced stability for efficient biocatalytic cascades and storage, after treated with hash conditions involving high temperature, UV light, and protease. Moreover, the hydrogel microparticles allow easy recycling, and good reusability after repeatedly treated with high temperature (80 ° Celsius). This work provides a simple and efficient strategy to create multiple-enzyme-loaded microcarriers with good stability and reusability for biocatalytic cascades.

Microfluidic fabrication of hydrogel microparticles with MOF-Armoured multi-enzymes for cascade biocatalytic reactions

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.