Background and Novelty: Collection and washing of Nk T-cells has presented many challenges with traditional rotor centrifuge methods. The challenges for the bottle centrifuge include product loss -reduced recovery, lengthy process, and potential for contamination in a open systems. The Single use centrifuge address all these challenges. The UniFuge was first used for cell collection and vaccine production 12 years ago. Ten years ago, we started to optimize the collection and washing stratagies of stem cells and lymphocycytes. Two years ago, we started to collect and wash NK T- cells. These NK T-cells presented challenges and required additional parameter optimization. In this presentation, we will look at parameters: feed pump and viability, g force and viabilty, washing volumes, and parameters for overall increased recovery. Experimental Approach: We will examine the collection and cell washing of NK T-cells in both a small scale 5 liter bioreactor and in a large Scale 50 liter bioreactor utilizing two different scaled Single Use Centrifgues- UFmini and UniFuge. Both Single use centrifuges will use the same process parameters scaled 1:5. Overall recovery and viability will be determined by cell counting. Results and Discussion: Through the optimization of process parameters, we have demonstrated effective washing of cells, and achieving over all cell recovery greater than 95%.

Concentration, washing, media exchange of Cart T cells for 5-100 liter bioreactor utilizing  single use centrifuges- UFmin and UniFuge.

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.