Taking advantage of outstanding excellent target recognition accuracy and trans-cleavage ability, CRISPR/Cas12a system provides an alternative opportunity for designing fluorescence biosensors. To fully exploit the potential of the assay, we introduce here a method for stabilizing enzyme activity. Metal–organic frameworks (MOFs) are proposed to protect Cas12a from harsh environments. Herein, Cas12a surface-absorbed on hydrophilic MAF-7 can retain high activity and a detecting element termed Com was developed. Due to the high stability of our proposed element, Com shows excellent performances for nucleic acid detection with a detection limit of as low as 0.04 nM, yielding a detection limit of 1 copy for SARS-CoV-2 RNA. Considering its good stability, this combination provides a viable MOFs-based immobilization strategy to improve biological activity.

Enhanced activity of CRISPR-Cas12a immobilized on hydrophilic metal: Organic frameworks for SARS-CoV-2 detection

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