The exposure of ecologically critical invertebrates to pharmaceutically active compounds in aquatic environments has been one of the major concerns over the past decade which also adds serious risk to the aquatic ecosystem. However, the metabolic level perturbations in invertebrates in response to sub-lethal doses of pharmaceuticals are still rarely studied, especially in the marine coastal environment. In this study, the diclofenac regulation of southern hard clam Mercenaria campechiensis metabolites under different time points and concentrations was investigated using NMR-based metabolomics. As a result, the clam metabolic profile perturbations were observed under both the low and high concentrations of diclofenac exposure in one week according to the principal component analysis (PCA) and partial least square – discriminant analysis (PLS-DA), however, the potential influenced metabolic pathways were distinctly different. The low-concentration group showed significant taurine upregulation which indicated self-protection from osmotic stress. However, the metabolites succinate, alanine, and glutamate were significantly upregulated in the relatively high concentration of diclofenac, which was a sign of anaerobic activities. The metabolic profile perturbations in Week 2 showed high similarity in both low and high-concentration groups, and the osmotic protectants betaine and taurine were significantly downregulated. The study indicated the early markers of diclofenac exposure in M. campechiensis which provided pioneering results for monitoring the toxicity of pharmaceuticals to marine coastal water.

Metabolomics study on southern hard clam (Mercenaria campechiensis) response to diclofenac exposure

Where thousands of chemistry professionals meet to share ideas and advance scientific and technical knowledge. Spring 2023 abstracts, posters, and presentations will launch at the end of March.

ACS Spring 2023

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.