β-Galactosidase (E.C. 3.2.1.23) is a crucial enzyme that hydrolyzes D-galactosyl residues like lactose or β-galactose comprising chromogenic or fluorogenic substrates from oligosaccharides and polymers. β-galactosidase has been employed in many food industries to hydrolyze lactose, enhance digestibility, sweetness, solubility, and flavor of dairy products. Carbon dots (CDs) are zero-dimensional carbon-based spherical nanoparticles with diameters less than 10 nm. In this paper, we employed the CDs in the storage of the β-galactosidase enzyme. We are all aware of the fact that the isolated enzyme needs to be stored in the freezing environment at the temperature range of -20 oC to -80 oC. The capability of storing of the enzyme in their near native environments will bring transformative impact in its future use and the research. In this work, we showed that β-galactosidase can be stored in the room temperature using the CDs. Our technique is facile, cost effective, and reproducible. We hope our study will open many facets of the use of the β-galactosidase enzyme in near future.

Use of carbon dots nanoparticles in the storage of hemoglobin and βeta-galactosidase enzyme

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