Before biological molecules such as proteins, peptides, lipids, and metabolites can be measured by mass spectrometry (MS), they must first be ionized and transferred into the gas phase. Electrospray ionization has become a preferred method as it is a “soft” ionization technique. This is because it can ionize large biomolecules without fragmenting them. Lower flow rates and decreasing post-column diffusion increases MS sensitivity, enabling single cell analysis. To obtain the high sensitivity, the Kelly group has developed an approach for fabricating sensitive columns with integrated emitters. Silica capillaries are constricted to a point with a laser puller, etched open with hydrofluoric acid, and fritted for packing. Integrating the emitter with the column should cause decreased loss and increased low flow stability and sensitivity.

Columns with integrated emitters for low-flow nanoelectrospray ionization

Where thousands of chemistry professionals meet to share ideas and advance scientific and technical knowledge. Spring 2023 abstracts, posters, and presentations will launch at the end of March.

ACS Spring 2023

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.