Identification of major xenobiotic metabolites is a requirement for the registration of agrochemicals around the world. This effort often requires trace-level identification of metabolites from complex environmental matrices. Decreasing application rates, an increasing number of studies, and a lack of extraction/purification methods often challenge initial mass spectrometry-based observation strategies. To face this challenge, we are developing and utilizing additional approaches to generate metabolites, which we term non-traditional metabolite generation efforts.  Plant metabolism of xenobiotics is regularly studied in mature tissue. However, there are many hurdles that researchers encounter when designing these experiments, including the maturing time, formulation approaches, and tissue sampling. Those challenges often limit the execution of early probe work. One way to simulate this mature plant metabolism is to utilize in vitro plant cell cultures to mimic the metabolism pathway.  This presentation will describe the experimental details and identification results from the application of an agrochemical to plant cell cultures such as wheat, soybean, or blackgrass cells. Typically, the application solution will include a blended stable isotope strategy to increase the identification rate. This approach combined with high-resolution MS and MS<sup>n</sup><sup> </sup>can provide a highly selective method to detect and identify these plant metabolites. The use of these plant cell cultures enables probe metabolism work to support later whole plant metabolism studies.

Agrochemical metabolite identification aided by plant cell culture experiments

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