We describe the fabrication and testing of a ssDNA aptamer-based electrochemical biosensor for detection of p57 or Major Soluble Antigen (MSA). Fabrication of the biosensor was accomplished by screen printing the electrode with a mix of graphite and carbon ink and painting Ag/AgCl paste to create the reference electrode. A high-affinity aptamer was then immobilized onto the electrode using EDC-NHS coupling. Electrode reproducibility was tested across multiple batches before and after surface modification using cyclic voltammetry. The red-ox properties were also characterized using different scan rates. The quantitative detection of the protein was based on the hindrance of the redox reaction of potassium ferrocyanide on the electrode surface due to the aptamer binding of the target protein. A rapid sensor for onsite detection of <i>Renibacterium salmoninarum</i> in water or in infected salmonids could be applied as an early warning for disease outbreaks and as an epidemiological tracking tool, significantly improving aquaculture management.

Fabrication and testing of ssDNA aptamer-based electrochemical sensor for the detection of <i>Renibacterium salmoninarum</i>

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Fabrication and testing of ssDNA aptamer-based electrochemical sensor for the detection of Renibacterium salmoninarum