Natural occurred novel citrus with pleasant flavor are precious to citrus industry, which could be applied as important gene donor, pollen parents or essential oil supplier plant material. Mangshanyegan (Citrus mangshanensis), C. reticulata ‘chachiensis’ and a brown ‘Zong Cheng’ navel orange (ZC) (C. sinensis Osbeck) are novel and potentially highly profit with pleasant odor. In the study, directional difference test and flash profile analysis was applied to investigated the significant differences in aroma sensorial attributes of the novel citrus fruits, combined with metabolomic analysis on volatile profiles throughout fruit development by using Gas chromatography-mass spectrometry (GC-MS), odor active substances identification via solvent-assisted flavor evaporation (SAFE), aroma extract dilution analysis (AEDA) on Gas chromatography-Olfactometry (GC-O), followed by omission experiment. As the result, β-myrcene, valencene and trans-β-ocimene was found as characteristic aroma components to ZC, which was more preferred by consumers. Finally, integrated transcriptomic analysis and gene characterization screened out two genes of TPS18 and LO1, which play important roles in valencene and linalool oxidates biosynthesis, respectively. The study lay a foundation for the flavor citrus breeding by identify the specific metabolites that satisfying consumers’ preference, and will benefit the future regulatory mechanism research on flavor related volatiles biosynthesis in citrus.

Omics and sensorial analysis combinedly revealed characteristic aroma and consumer preference of novel citrus

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