Anthracene is a well-known DNA intercalator which can help to localize DNA alkylating agents at the preferred site of action providing efficient inter-strand cross-link. We synthesized and characterized an anthracene analog, 1a which is a coupling product of an anthracene moiety and a bifunctional benzene derivative. Cross-linking efficiency of 1a was investigated with radio labeled 49-mer DNA duplex upon photoirradiation by RPR-100 Photochemical Reactor. DNA inter-strand cross-link (ICL) yield was investigated via denaturing polyacrylamide gel electrophoresis (PAGE) with phosphorimager analysis. The analog, 1a was found to improve cross-linking efficiency significantly compared to control in the time-dependent and concentration-dependent studies under UV irradiation. Subsequently, we will improve the ICL efficacy by structure modification.

Cross-linking efficiency of photo-active bifunctional Anthracene analog

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