Biomass DNA materials have recently gained traction due to declining DNA costs and their relatively underexplored applications at bulk scales. Typically derived from sheared chromosomal DNA, current methods cannot simply control the incorporation of functional sequences at scale. Due to the demand for nucleic acid therapeutics, bioreactor based production has tremendously increased the yield of plasmid DNA to &gt; 1 g of purified plasmid DNA (pDNA) per liter of culture fluid. In this work we discuss reverse engineering industrial scale fermentation and purification strategies in combination with new chemical cross-linking techniques to access gram scale amounts of pDNA which will act as precursors to DNA hydrogel materials with controlled sequences.

Scaling down bioreactor processes to create unimolecular DNA synthons for biomaterial applications

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