Mass spectrometry imaging (MSI) is a powerful technique for studying the localization of lipids, metabolites, and proteins in biological samples. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that relies on localized liquid extraction of analyte molecules from the sample into a liquid bridge formed between two glass capillaries. The extracted analytes are transferred to a mass spectrometer inlet and ionized by electrospray ionization. Nano-DESI enables quantitative imaging of biomolecules in fully hydrated samples with minimal or no sample pre-treatment. Recent developments in the nano-DESI MSI instrumentation have enabled quantitative imaging of lipids, metabolites, and proteins in tissues with high sensitivity and spatial resolution down to 8-10 microns. This capability enables accurate measurement of chemical gradients in biological tissues. When combined with other imaging modalities, MSI provides insights into cellular communication and biochemical pathways in biological systems.

Bringing ambient nano-DESI mass spectrometry imaging to the single-cell level

Where thousands of chemistry professionals meet to share ideas and advance scientific and technical knowledge. Spring 2023 abstracts, posters, and presentations will launch at the end of March.

ACS Spring 2023

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.