A critical component of any sensitive detection assay is a signal amplification step. Polymerization reactions intrinsically amplify an initiator signal because they form long polymer chains from the initiation event. Fluorogenic polymerization allows for visible, real-time monitoring of the signal amplification through fluorescent polymers that only form in the presence of the initiator, which is a model analyte.<br/>Reversible addition-fragmentation chain-transfer (RAFT) polymerization is a beneficial polymerization technique because it is controlled with living character, which allows for expressed fluorescence to track with analyte concentration over multiple ranges of magnitude. Additionally, it is sensitive to low concentrations of radicals and compatible with enzymatic degassing, permitting the development of an open-air reaction. Finally, the reaction is robust because it is activated by visible blue light, which allows for specific initiation of the polymerization and minimizes side reactions. Experiments to improve the compatibility of the fluorogenic RAFT polymerization with enzymatic deoxygenation, as well as continued optimization, will be discussed.

Open-vessel optimization of fluorogenic reversible addition fragmentation chain-transfer (RAFT) polymerization

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