Acetylcholine (ACh) plays a critical role in learning, memory, human health, attention, sleeping, and consciousness. Developing electrodes for detecting and quantifying ACh is critical. However, electrochemical detection of ACh is challenging due to its lack of redox activity. In this work, we present an ion selective electrode (ISE) for the selective and quantitative detection of acetylcholine. The electrode was fabricated based on electrodeposition of a carbon electrode with poly(3,4-ethylenedioxythiophene) (PEDOT) followed by drop-casting of an ionophore-doped poly(vinyl) chloride (PVC) membrane. An increase in capacitive current was observed during electrodeposition of PEDOT. The PEDOT layer was further characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) showing uniform deposition of PEDOT on carbon electrode. An increase in current was observed in cyclic voltammetry after adding ACh comparing to the blank. The current increased in a linear manner. The modified carbon electrode showed a wide linear range, great selectivity towards ACh detection, and good stability after 2-month storage. This surface modification strategy has the potential to be applied to microelectrodes for <i>in vivo</i> study in the future. This work also paves the way for future applied research to study neurological disorders using ACh as a biomarker.

PEDOT / PVC-modified amperometric carbon electrodes for acetylcholine detection

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