There is increased demand for point of care (POC) diagnostic systems, especially in developing countries. POC detection allows for rapid detection and monitoring of diseases, and in many cases does not require trained personnel to administer. Early disease detection and monitoring allows more efficient treatment and thereby more positive outcomes. Microfluidic paper-based analytical devices (µPADs) offer many advantages when developing POC diagnostics, including portability, cost efficient manufacturing, and adaptability for many purposes. With these advantages in mind, a POC detection system for trypsin was developed combining µPADs and engineered nanoparticles. The nanoparticles were designed to elicit a signal in the presence of typrin, which was quantified on the µPADs. The system was able to detect increasing concentrations of trypsin in buffer and multiple biological matrices, while showing no response in the presence of the non-specific blood protein, thrombin. This work lays the foundation for further POC diagnostic development.

Point-of-care trypsin detection with nanoparticle µPAD system

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