Parabens are commonly used as preservatives in regularly used topical products, but their safety is under discussion since small amounts of paraben have been found in tumor tissue. Mononitroparaben causes cell death in melanoma cells with an LC<sub>50</sub> value of 7.02mM after twelve hours of treatment. This study focused on how mononitroparaben (pictured in Figure 1) affects the cellular lipid content during induced cell death.<br/><br/>The experiment was conducted by growing the M624 melanoma cells, dissolving the paraben, and then leaving 0 mM, 5 mM, and 10 mM concentrations of paraben on the melanoma cancer cells for twelve hours. The paraben was then removed from the cells, and the cells were lysed. Colorimetric cholesterol assays and ceramide dot blot assays were completed to determine the changes in cellular cholesterol and ceramide content and the role of cellular lipids in cell death signaling.

Effects of mononitroparaben on lipid content of melanoma cells

Where thousands of chemistry professionals meet to share ideas and advance scientific and technical knowledge. Spring 2023 abstracts, posters, and presentations will launch at the end of March.

ACS Spring 2023

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.