Atrazine is a pesticide of the triazine class and is one of the most used pesticides in North America. The effects of atrazine exposure on crayfish are of interest because crayfish are a keystone species. Atrazine has many negative health effects such as disrupting regular hormone function, birth defects, and DNA damage in the hepatopancreas. Furthermore, atrazine can cause metabolic changes that diminish the ability for crayfish to localize an odor source, making it difficult to locate food and find a mate. Exposure to atrazine also affects the expression and activity of the detoxification enzymes cytochrome P450 and glutathione-S-transferase. To further explore the effects of atrazine exposure, crayfish (<i>Faxonius virilis</i>) were exposed to atrazine for four days. Liquid chromatography-mass spectrometry (LC-MS) was used to standardize the atrazine solutions to which crayfish were exposed. The morphology of the hepatopancreas of atrazine-exposed crayfish was examined; lobule lumens were dilated, and tubule tissue was degenerated and contained an increase in vacuoles. We aim to quantitate atrazine accumulation in the hepatopancreas tissue to assess potential correlation between behavioral and physiological effects of atrazine exposure with atrazine accumulation. We developed and validated an extraction and LC-MS analysis method for quantitation of atrazine in the hepatopancreas tissue by performing a recovery test that yielded an average percent recovery of 93.96%. The validated method will be used to measure atrazine in the hepatopancreas.

Quantitation of atrazine for toxicological studies in the keystone species <i>Faxonius virilis</i>

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Quantitation of atrazine for toxicological studies in the keystone species Faxonius virilis