The use of silk micro/nanoparticles as drug delivery devices has gained attention due to the desirable biodegradability and biocompatibility of silk fibroin. Here, we report a method for preparing silk microparticles that can be non-invasively tracked <i>in vivo </i>using the bioluminescent enzyme Nanoluciferase (NLuc). NLuc enzyme was covalently attached to silk microparticles via strain-promoted alkyne-azide cycloaddition, and long-term stability and light output was compared to silk microparticles with non-specifically bound NLuc. Furthermore, methods to co-deliver NLuc and its substrate furimazine using silk microparticles are described.

Development of bioluminescent silk microparticles

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