An expression cell line for RecA from Mycobacterium tuberculosis is needed to confirm observations made with RecA from Escheridia coli that suggest a direct role for the protein in the antibiotic resistance development. Plasmids containing ampicillin resistance and a gene coding for either full-length MtRecA (as it exists in M. tuberculosis) or mature MtRecA (with the region coding for the self-splicing intein omitted) were transformed into DH5-α cells to set up a sustainable source of both plasmids. Plasmid isolates were then transformed into a rhamnose inducible KRX expression cell line. Plasmid isolates were confirmed through restriction digests. The presence of ampicillin resistant expression cells suggest successfull transformation, but RecA protein has not yet been expressed and purified.

Engineering an expression cell line for Mycobacterium tuberculosis

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