Lipoprotein metabolism disorders are key catalysts to the appearance of CVD (cardiovascular disease), the leading cause of death in America. In response to this, there is considerable interest in the discovery of new methods that can analyze lipoprotein complexes to prevent CVD. We are using anion-exchange High Performance Liquid Chromatography (AEX-HPLC) developed by Yuji Hirowatari in 2003 to analyze lipoproteins (HDL, LDL, VLDL, and chylomicrons) who continues to expand upon this technology; however, no other research has proposed any modification to further improve AEX-HPLC. Our novel approach uses a lipid-specific fluorescent probe, NBD C6-ceramide, in conjunction with AEX-HPLC to further elucidate the human serum profile with methodology that is facile and efficient. We perform various experiments to sufficiently support our methodology’s value for further HPLC-based lipoprotein profiling. We achieve this by generating a highly selective and sensitive fluorescent serum separation that is potentially quantitative. We expect this fluorescent anion-exchange methodology to contribute to the diagnosis, treatment, and prevention of CVD.

Lipoprotein profiling via fluorescence anion-exchange high performance liquid chromatography

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