Several new aromatic fluorescein diesters were synthesized and added to an existing library of fluorogenic compounds used in the enzymatic assay of esterases. These aromatic fluorescein diesters were synthesized via a nucleophilic substitution reaction of varying aromatic carboxylic acids with bis(chloromethyl)fluorescein. The newly synthesized products were characterized using HNMR, CNMR, and HRMS, confirming that the desired diester was obtained. These new diesters were used along with existing compounds in our library to study substrate affinity in the evaluation of esterase enzymatic activity.

Synthesis of aromatic fluorescein esters for biochemical analysis

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