Aptamers are identified based on their ability to bind to specific molecules. However, upon binding, aptamers can also impact the properties of their ligands. Recently we have reported the ability of a flavin-binding RNA aptamer to shift the reduction potential of the bound flavin. To explore if DNA aptamers have similar abilities, we have undertaken a series of spectroscopic assays on recently reported DNA aptamers for flavin adenosine dinucleotide (FAD) and riboflavin. Flavin binding has been assessed in the presence of different divalent metal ions and under a range of pH conditions. The reduction potential of the bound flavin is being analyzed using a previously reported xanthine oxidase enzymatic assay, as well as by spectroelectrochemistry. The results of these studies and the potential impact of reduction potentials shifts by DNA aptamers in future applications will be discussed.

Determining the reduction potential shifting ability of flavin-binding DNA aptamers

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