UHRF1 and UHRF2 proteins are multi-domain epigenetic proteins. These two proteins share many similarities in sequence and structure. Previous research in our lab has shown that these proteins bind to H3K9me3 tail peptides. The purpose of our study is to measure UHRF1 and UHRF2 protein interactions with nucleosomes, which are more biologically relevant binding partners. These experiments were conducted by nucleosomes pull-down assays. The procedures were optimized using H3K9me3 and unmodified nucleosomes, followed by testing novel nucleosome binding partners for UHRF1 and UHRF2.

Characterizing UHRF1 and UHRF2 interactions with nucleosomes

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