Author Listing - Lead/ Submitting Author (we will be communicating with this individual): Name, Institution, City and State where the work was done. Co-authors will be added in a separate section.
Name: João Henrique Rissato. Institution: Instituto do Coração da Faculdade de Medicina da Universidade de São Paulo. City: São Paulo. State: São Paulo.

Main Body of the Abstract
Introduction: The gastrointestinal tract may be affected by deposits in the parenchyma and also due changes in the autonomic nervous system. However, heart failure also may cause edema and hypoperfusion leading to an increased gut permeability and predisposes to intestinal microbiome disturbances.
Objective: this study aimed to identify the intestinal microbiome composition in patients with transthyretin amyloidosis with and without cardiac involvement and control group and correlate with echocardiographic parameters and cardiac biomarkers such as troponin and BNP.
Methods: 60 adults were included: 20 with cardiac involvement defined by a ventricular septum thickness > 12 mm (G1), 20 without cardiac involvement (G2) and 20 controls (G3). The microbiome profile was obtained by sequencing the 16S rRNA gene (Illumina® Miseq).
Results: among the 40 patients (G1 and G2), 85% had the hereditary form and 15% the wild form. The most prevalent mutation was Val142Ile (52,9%), followed by Val50Met (17,6%). The differentially abundant bacterial genera in G1 and G2 were: Streptococcus, Lachnospiraceae and Selimonas, while G3 presented a higher relative abundance of Metahanosphaera (figure 1). Butyricoccaceae and Streptococcus were associated with higher levels of troponin I, while the genus Hungatela was associated with higher circulating levels of BNP (p<0.05). Larger left atrial volumes were associated with Lachnospiraceae and higher cardiac mass indexes were associated with Butyricoccaceae (p<0.05).
Conclusions: patients with amyloidosis presented a particular intestinal microbiome profile when compared to control group and there was correlation with echocardiographic  parameters and biomamarkers of heart failure and necrosis.

**Author Listing - Lead/ Submitting Author (we will be communicating with this individual): Name, Institution, City and State where the work was done. Co-authors will be added in a separate section.**
Name: João Henrique Rissato. Institution: Instituto do Coração da Faculdade de Medicina da Universidade de São Paulo. City: São Paulo. State: São Paulo.

**Main Body of the Abstract**
**Introduction**: The gastrointestinal tract may be affected by deposits in the parenchyma and also due changes in the autonomic nervous system. However, heart failure also may cause edema and hypoperfusion leading to an increased gut permeability and predisposes to intestinal microbiome disturbances.
**Objective:** this study aimed to identify the intestinal microbiome composition in patients with transthyretin amyloidosis with and without cardiac involvement and control group and correlate with echocardiographic parameters and cardiac biomarkers such as troponin and BNP.
**Methods**: 60 adults were included: 20 with cardiac involvement defined by a ventricular septum thickness > 12 mm (G1), 20 without cardiac involvement (G2) and 20 controls (G3). The microbiome profile was obtained by sequencing the 16S rRNA gene (Illumina® Miseq).
**Results:** among the 40 patients (G1 and G2), 85% had the hereditary form and 15% the wild form. The most prevalent mutation was Val142Ile (52,9%), followed by Val50Met (17,6%). The differentially abundant bacterial genera in G1 and G2 were: _Streptococcus_, _Lachnospiraceae_ and _Selimonas,_ while G3 presented a higher relative abundance of _Metahanosphaera_ (figure 1). _Butyricoccaceae_ and _Streptococcus_ were associated with higher levels of troponin I, while the genus _Hungatela_ was associated with higher circulating levels of BNP (p<0.05). Larger left atrial volumes were associated with _Lachnospiraceae_ and higher cardiac mass indexes were associated with _Butyricoccaceae_ (p<0.05).
**Conclusions**: patients with amyloidosis presented a particular intestinal microbiome profile when compared to control group and there was correlation with echocardiographic  parameters and biomamarkers of heart failure and necrosis.

Analysis of the intestinal microbiome in patients with transthyretin amyloidosis with and without cardiac involvement and its correlation with ecocardiographic parameters and biomarkers.  OC4 (#330)

We invite you to attend the XIX International Symposium on Amyloidosis to be held in Rochester, MN.

This symposium is organized as a hybrid meeting (with the focus on in-person) held from Sunday, May 26 through Thursday, May 30, 2024.

Together with the ISA Board and members of Mayo Clinic, a very attractive scientific hybrid program is being planned. The program will cover a variety of types of systemic amyloidosis: ATTR (hereditary and wild-type), AL (light-chain), AA and less frequent forms. There will be key lectures, specific symposia including those company sponsored, oral presentations and poster sessions of accepted abstracts.

COURSE DESCRIPTION
This program will provide a comprehensive review of state-of-the-art education in systemic amyloidosis: basic science, pathophysiology, diagnosis, current and emerging treatments. Scientific presentations will include competitive oral and poster abstracts. The rapid evolution of scientific discovery and expanding treatment options has created tremendous interest in the field and this meeting will fill and important gap in educating scientists and clinicians.